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The disparity of chain length distribution patterns and carbon isotopic compositions between different fatty acid purification procedures

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Authors:Zhao Jiangtao; Liu Weiguo; An Zhisheng
Author Affiliations:Primary:
Chinese Academy of Sciences, Institute of Earth Environment, Xi'an, China
Volume Title:Yankuang Ceshi Rock and Mineral Analysis
Source:Yankuang Ceshi = Rock and Mineral Analysis, 32(1), p.101-107. Publisher: Dizhi Chubanshe, Beijing, China. ISSN: 0254-5357
Publication Date:2013
Note:In Chinese with English summary. 23 refs.; illus., incl. 2 tables
Summary:Saturated fatty acids and their isotopic composition are important proxies for reconstructing the paleoenvironment and paleoclimate. There are several extraction and purification procedures for n-fatty acids based on different principles. However, a comparison test between different processes has not been reported. In this article, the correlation of fatty acid and its isotope compositions purified using different processes directly impacted the proxies' application, especially when comparing the reconstruction results from different regions in global change research. Two common fatty acid purification procedures were used to purify the lipids of fatty acid standards, plant, and peat samples. For the standards, the recovery rates of these two procedures were all greater than 85%, which indicates that these two methods are reliable for processing n-fatty acids. However, for the plant and peat samples, Procedure 1 obtained a relatively large amount of shorter chain length n-fatty acids. According to our results, Procedure 2 obtained almost all the free fatty acids, while Procedure 1 can obtain both the free and ester state n-fatty acids in the samples. Since the free state fatty acids and esters state fatty acids in the sediment can transform into each other, using Procedure 1 to analyze the total fatty acids in the sediment sample was more appropriate. Alternatively, we can use Procedure 2 after the ester-state fatty acids in the lipids were released as free state acids by saponification.
Subjects:C-13/C-12; Carbon; Carboxylic acids; Chemical analysis; Chromatography; Distribution; Fatty acids; Gas chromatography; Isotope ratios; Isotopes; Methods; Organic acids; Organic compounds; Patterns; Purification; Sample preparation; Stable isotopes
Record ID:740928-17
Copyright Information:GeoRef, Copyright 2021 American Geosciences Institute.
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